#3276 LUPUS NEPHRITIS: THE INTERPLAY BETWEEN SENESCENT B AND NAÏVE T LYMPHOCYTES

Abstract Background and Aims Systemic Lupus Erythematosus (SLE) is characterized by a shift to senescent B cells, namely, switched memory (SM) (CD19+IgD-CD27+) exhausted-double negative (DN) (CD19+IgD-CD27-) subtypes. The aim of this study was evaluate the impact T cell immunity in distinct - pattern SLE. Method A wide range exhaustion related lymphocyte surface molecules, including CD45RA, CCR7, CD31, CD28, CD57, on cells CD27 IgD analyzed flowcytometry naïve, active subtypes were determined 31 patients with inactive Nephritis (LP). Results Comparison SM DN subpopulations revealed significant correlation naïve “unexperienced” CD4 CD8 compartment cells. Indeed, appeared even stronger regarding Recent thymic emigrants (RTE) CD4CD31+ (r=0.575), CD4+ Central Memory (CM) (CD45RA-CCR7+) (r=0.546), CD4CD45RA+CD28+(r=0.556), CD4CD28+CD57- (r=0.621), CD4CD45+CD57- (r=0.544), CD4CD45-CD57- (r=0.65), CD8CD28+CD57- (r=0.596), CD8CD45-CD57- (r=0.556) while weak yet their Naïve (CD45RA+CCR7+) (r=0.432), CD8CM(CD45RA-CCR7+) (r=0.474), RTE CD8CD31+ (r=0.461), CD8CD28+CD57+ (r=0.442). Less apparent phenomenon between CM (r=0.381), CD4CD45RA+CD28+ (r=0.504), (r=0.587), (r=0.487), CD4CD28+CD57+ (r=0.491), (r=0.419), (r=0.397), Naive(CD45RA+CCR7+) (r=0.366), (r=0.363), (r=0.483), (r=0.444), CD8CD45-CD57+ (r=0.384). Conclusion predominant senescent/exhausted lymphocytes SLE LN may have direct subpopulations.